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Please note that all translations are automatically generated. Click here for the English version.
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02:11 min
May 19th, 2023
DOI :
10.3791/200189-v
Transcript
要使用DIC软件执行数字图像相关(DIC),请将从纯粹的测定记录中获得的图像导入DIC软件,最好是TIF文件格式。为了优化相关性,请选择微分和选项,该选项跟踪新图像相对于最后一个图像的变形。选择 31 x 31 像素、步长为 20 像素的子集大小。
绘制出所选单个像元的感兴趣区域。对于不规则形状(如单元格),请使用多边形蒙版映射单元格的几何图形。选择映射单元格内的任意点以跟踪变形。
映射后,选择要分析的细胞上的特定点,如细胞核或细胞质,方法是单击添加应变片并在定义的
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