To begin, select the appropriate workflow option from the MVS software. Follow the workflow prompts to confirm the electrical connection between the holder and the heating e-chip by loading the calibration file and performing a device check. After connecting the microscope to the MVS software, center the sample ROI in the field of view.
Access the Temperature Control Settings by clicking the Experiment button and Manual Control mode. Then, set the ramp rate to 25 degrees Celsius per second and the target to 200 degrees Celsius. Click on Apply to start the experiment.
After reaching 200 degrees Celsius, set the ramp rate to 10 degrees Celsius per second. Adjust the target to 600 degrees Celsius and click Apply. After the set temperature of 600 degrees Celsius is reached, change the ramp rate to 2 degrees Celsius and the target to 800 degrees Celsius.
Click on Apply to start the experiment. Once done, open the Analysis Software to review the session. In the timeline, plot the temperature, template morphing factor, dose rate, and cumulative dose.
Export images and movies using the Publish option with or without the dose map overlays. The heating experiment performed using a representative nanocatalyst sample gold on iron oxide showed that at elevated temperatures, the gold nanoparticles within the gold on iron oxide migrated along the surface of the iron oxide support and centered to form larger particles. An in-situ heating experiment recorded a series of TEM snapshots of a porous region within a gold on iron oxide nanocatalyst at various time points.
The coordinated drift of the sample increased with the increased temperature from a rate of 9 to 62 nanometers per minute, and began to decrease towards leveling off with the constant temperature. The MVS software stabilized the particle in the field of view throughout the entire temperature ramp profile, enabling high resolution imaging.
