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02:46 min
April 21st, 2023
DOI :
10.3791/200196-v
Transcript
通过从小鼠解剖血管纹来准备免疫染色,并将组织添加到含有200微升4%多聚甲醛的1X PBS中的24孔板中。将组织在室温下孵育20分钟。在低转速下在轨道振动台上短时间洗涤两次 1X PBS。
取出PBS,然后在室温下在300微升PBST溶液中进行透化和封闭至少一小时。接下来,用一抗在 4 摄氏度下对组织染色过夜。然后加入二抗,并在室温下以低RPM在轨道振荡器上孵育两小时。
盖上平板以保护荧光标记的二抗免受光照。接下来,沿着 25 mm 轴放置两条小胶条,仅比 18 x
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