Name: Preparing Zanba-Stir-Fried Tiebangchui (TBC) Sample for Processing Technology Optimization
Uploaded: 2023-05-12T13:20:00
Description: Watch this Scientific Journal Video about Preparing Zanba-Stir-Fried Tiebangchui TBC Sample for Processing Technology Optimization at JoVE.com

preparing zanba-stir-fried tiebangchui (tbc) sample for processing technology optimization

En metode for Zanba-stekt Tiebangchui-prosessoptimalisering

The dried root of Aconitum pendulum Busch and A. flavum Hand.-Mazz., one of the most famous Tibetan medicines, is called Tiebangchui (TBC) in Chinese1,2. The dried roots of TBC are helpful in dispelling cold and wind, reducing pain, and calming shock. It was recorded in the first volume of "Drug Standards (Tibetan Medicine) of the Ministry of Health of the People's Republic of China," which states that the dried roots of TBC are commonly used to treat rheumatoid arthritis, bruises, and other cold diseases3. However, the clinical therapeutic dose of TBC is similar to its toxic dose, and incidents of poisoning or death have been frequently reported due to improper use4. Therefore, reducing the toxicity and preserving the efficacy of TBC has become a research hot spot over the years.
In Tibetan medicine, processing is one of the most effective methods to attenuate the toxicity of TBC. According to "Processing specification of Tibetan medicine of Qinghai Province (2010)", the original herbs (TBC) should be placed in an iron pot and stir-fried with Zanba until the Zanba turns yellow, after which Zanba is removed and the herbs are dried in air5,6. However, no specific process parameters have been documented, which makes controlling the processing technology and the quality of Zanba-stir-fried TBC difficult. The CRITIC method is an objective weight method that can avoid fuzzification and subjectivity, and enhance the objectivity of weighing7. The Box-Behnken response surface method can directly reflect the interaction between each factor through polynomial fitting8. The combination of the Box-Behnken response surface and CRITIC method is commonly used to optimize processing technology to acquire the optimized processing protocol9,10. In this paper, a monoester-diterpenoid alkaloid (MDA) (benzoylaconitine) and two diester-diterpenoid alkaloids (DDAs) (aconitine, 3-deoxyaconitine) were used as evaluation indexes. CRITIC combined with the Box-Behnken response surface method was applied to optimize the processing technology of Zanba-stir-fried TBC and establish a standard processing method for clinical safe use.

Forfatter Spotlight: Optimalisering av prosesseringsteknologi for Tiebangchui med Zanba basert på CRITIC kombinert med Box-Behnken Response Surface Method  

Optimalisering av prosesseringsteknologi for Tiebangchui med Zanba basert på CRITIC kombinert med Box-Behnken Response Surface Method

The scope of this protocol lies in the proceeding of the Tibetan medicines. This staff aims to the optimize and standardize the processing technology of Zanba-stir-fried Tiebangchui. The combination of the Box-Behnken response surface and the CRITIC method is commonly used to optimize proceeding technology to acquire the optimized proceeding protocol.The optimized processing conditions of two centimeter Tiebangchui slice thickness three times more Zanba than Tiebangchui, a processing temperature of 125 degrees C and the 60 metres of stir-frying. By quantifying the specific processing parameters, this method will benefit the large-scale production and the quantity control of Zanba-stir-fried Tiebangchui. The standard Zanba-stir-fried processing technology of Tiebangchui can provide useful information and the guidance for the processing of other poisonous ethnical medicines.The optimized process is stable and feasible, laying a certain foundation for further investigation of Tiebangchui products and related Chinese medicine preparations. Our laboratory will pay more attention to cytochemical investigation to obtain the targeted quantity control compliance. In addition, the pharmacological comparison overall in Zanba-stir-fried Tiebangchui is unclear.Detoxification and evaluation of the efficacy, reservation effects in animal models will be the next objectives.

Watch this Scientific Journal Video about Preparing Zanba-Stir-Fried Tiebangchui TBC Sample for Processing Technology Optimization at JoVE.com

Preparing Zanba-Stir-Fried Tiebangchui (TBC) Sample for Processing Technology Optimization  

Klargjøre Zanba-Stir-Fried Tiebangchui (TBC)-prøve for optimalisering av prosesseringsteknologi

For å starte et enkeltfaktoreksperiment for å sammenligne Tiebangchui, eller TBC-skivetykkelse, for optimalisering av prosesseringsteknologi, utarbeide fem testgrupper som hver har 30 gram TBC, men forskjellig skivetykkelse. Deretter veier du 90 gram Zanba, som er tre ganger så mye som TBC i hver gruppe. Sett temperaturen og tiden for den automatiske stekemaskinen til henholdsvis 140 grader Celsius og 40 minutter.Etter at maskinen er varmet opp til den innstilte temperaturen, tilsett de veide mengdene TBC og Zanba i maskinen. Bruk deretter en høyhastighets smashing maskin, slip Zanba woke-stekte TBC i pulver. Deretter veier du to gram Zanba wokede TBC-pulver i en konisk kolbe.Til prøven i kolben, tilsett tre milliliter ammoniakkoppløsning etterfulgt av 50 ml av en blanding av isopropylalkohol og etylacetat, med et volumforhold på en er til en. Etter registrering av vekten av den koniske kolben med innholdet, ultralyd TBC-prøven i 30 minutter ved 220 volt og 40 kilohertz opprettholde en temperatur under 25 grader Celsius. Vei den koniske kolben med prøven igjen etter ultralydutvinningen.Kompenser for den tapte vekten ved å tilsette isopropylalkohol- og etylacetatblandingen nevnt tidligere, og filtrer prøveløsningen. Fordamp 25 ml av filtratet til tørrhet ved bruk av en roterende fordamper ved 40 grader Celsius. Oppløs deretter resten i fem milliliter av en løsning av 0,05 saltsyre i metanol.Filtrer løsningen gjennom et 0,2 mikron sprøytefilter, og analyser det ved å utføre væskekromatografi med høy ytelse, eller HPLC. Beregn innholdet i MDA og DDA i forskjellige prosesseringsprodukter i henhold til standardkurven. Beregn den omfattende poengsummen basert på resultatene via kritikermetoden.Etter metoden demonstrert ovenfor, sammenlign mengdene Zanba, behandlingstemperaturer og tider for optimalisering av prosesseringsteknologi. Resultatene av enkeltfaktortestene viste at de omfattende poengsummene for Zanba wokede TBC var høyest når den skivede tykkelsen er to centimeter, mengden Zanba er tre ganger, behandlingstemperaturen er 120 grader Celsius, og behandlingstiden er 60 minutter. Resultatene avslørte rekkevidden av skivetykkelse, mengde Zanba, prosesseringstemperatur og behandlingstid som skal brukes til fremtidige eksperimenter.

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Preparing Zanba-Stir-Fried Tiebangchui (TBC) Sample for Processing Technology Optimization

Optimization of Processing Technology for Tiebangchui with Zanba Based on CRITIC Combined with Box-Behnken Response Surface Method

The dried root of Aconitum pendulum Busch., called Tiebangchui (TBC) in Chinese, is one of the most famous Tibetan medicines. It is a widely used herb in ...