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Please note that all translations are automatically generated. Click here for the English version.
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05:38 min
May 26th, 2023
DOI :
10.3791/200204-v
* These authors contributed equally
Transcript
首先将组织放入无菌组织培养板中并除去多余的培养基。用无菌金属尺测量组织并拍照。向切割的纸巾中加入 3 毫升高级 DMEM/F-12 培养基,然后上下吸取纸巾进行清洗。
然后用三毫升PBS清洗组织。从组织培养板中吸出培养基,并使用无菌刀片和两毫升消化培养基将其切成小块。然后将组织收集到一个 50 毫升的管中,该管总共含有 5 毫升的消化培养基。
将试管在 37 摄氏度下孵育一小时。通过上下移液定期混合悬浮液。通过向试管中加入 5 毫升高级 DMEM/F-12 来灭活胰
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