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Please note that all translations are automatically generated. Click here for the English version.
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08:09 min
April 14th, 2023
DOI :
10.3791/200206-v
Transcript
首先,关闭房间灯。在 FlowView 软件的眼部面板下选择眼部。将立方体转塔更改为四个TRITC,并在软件中触摸屏控制器的背光下单击关闭触摸面板控制器。
然后关闭计算机屏幕。打开显微镜上黑布盖的正面。从黑匣子中取出装有胚胎的35毫米玻璃底培养皿,并将其放在显微镜载物台上。
然后打开荧光照明装置,并使用目镜识别感兴趣的胚胎。聚焦它。关闭黑布盖以保护样品免受光照。
打开计算机屏幕以访问控制显微镜的软件。在软件中将眼睛更改为LSM以进行图像采集。使用25倍放
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