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Please note that all translations are automatically generated. Click here for the English version.
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01:22 min
October 6th, 2023
DOI :
10.3791/200212-v
Transcript
首先取准备好的存档,新鲜组织或多聚甲醛固定的小鼠脑组织载玻片。用菱形笔切下薄片盖玻片,制成垫片。然后,用少量水、PBS 或强力胶将它们固定在载玻片上。
轻轻地将垫片放在组织的两侧。在使用前立即合并试剂来制备最终的胶凝溶液。从组织切片中取出多余的溶液后,将载玻片放入培养皿中。
将新鲜制备的冷凝胶溶液添加到样品中,并将混合物在4摄氏度下在纸巾上孵育30分钟,以使其扩散到组织中。小心地将第二个未涂层的载玻片放在组织和凝胶溶液上,避免气泡。通过在潮湿环境中在37摄氏度下孵育
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