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Please note that all translations are automatically generated. Click here for the English version.
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01:39 min
October 6th, 2023
DOI :
10.3791/200213-v
Transcript
首先,用聚合的存档新鲜组织或多聚甲醛固定的小鼠脑组织拍摄载玻片。戴上护目镜,用剃须刀片将胶凝室的两个载玻片分开。修剪组织周围的空白凝胶以最小化体积,并确保不对称切割以跟踪均质后凝胶的方向。
用剃须刀片轻轻地从载玻片上提起含有凝胶的组织。将其转移到两毫升离心管中。用预热至80摄氏度的均质缓冲液将试管填充到顶部。
然后将样品在 80 摄氏度下振荡孵育 8 至 60 小时。将离心管的内容物倒入六孔塑料细胞培养板的单孔中,并用移液管除去变性缓冲液。要从水凝胶中完全去除剩余的
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