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Please note that all translations are automatically generated. Click here for the English version.
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05:24 min
April 14th, 2023
DOI :
10.3791/200244-v
Transcript
首先,将8至10周龄的麻醉C57黑6小鼠放在手术台上。使用钝镊子,拉出舌头并握住它。将喉镜插入口腔并观察声带。
将导丝插入 20 号静脉导管。然后轻轻地将导管推入气管,直到其翼部与鼻尖齐平。将鼠标连接到呼吸机,并将其置于加热灯下的仰卧位。
将直肠温度保持在37摄氏度。剃须和消毒手术区域后,在左右腹股沟区域做一个1厘米的皮肤切口,以进入股动脉。然后对股动脉远端进行消毒和结扎,并滴一滴利多卡因。
在股动脉近端放置一个夹子,并在夹子远端的动脉上做一个小切口。将
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