Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
134 Views
•
02:37 min
May 12th, 2023
DOI :
10.3791/200253-v
Transcript
首先准备聚合页面凝胶三明治。取下夹子和纸胶带层,慢慢取下梳子。用蒸馏水彻底冲洗孔。
将凝胶夹层正确放置在垂直电泳装置中。用TBE缓冲液填充顶部和底部储液罐。通过在 50 瓦的功率下进行凝胶电泳预运行至少 30 分钟来预热板。
同时,将干燥的样品重悬于5微升变性凝胶上样缓冲液中。接下来,使用装有TBE缓冲液的小注射器将尿素从凝胶孔中冲洗出来。现在将样品加载到干净的孔中,注意加载的气味。
以 50 瓦的功率运行凝胶两个小时,或直到溴酚蓝染料耗尽凝胶的 2/3
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved