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Please note that all translations are automatically generated. Click here for the English version.
231 Views
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00:52 min
June 23rd, 2023
DOI :
10.3791/200267-v
Transcript
首先将一到五天大的雌性处女蝇与一到七天大的成年雄性苍蝇杂交,以产生具有所需基因型的后代。为此,请将苍蝇放在带有餐盖的苍蝇笼中。使用 10 到 15 个女性处女和 5 到 10 个男性来获得最佳产量。
将装有苍蝇的苍蝇笼在25摄氏度下孵育。每天更换餐帽,以防止幼虫过度拥挤,从而降低解剖大脑的质量。如果餐盖有超过30只幼虫,请将其分成两半,并用切成两半的新鲜餐盖代替一份。
将餐盖与幼虫一起在25摄氏度下孵育,直到幼虫达到所需年龄。
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