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Please note that all translations are automatically generated. Click here for the English version.
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06:28 min
August 11th, 2023
DOI :
10.3791/200271-v
Transcript
首先,从安乐死的小鼠幼崽中分离出大脑,并将其放入含有具有EBSS和2X PSN的冷MEM的三厘米培养皿中。在解剖镜下,分离大脑半球,切除并丢弃中脑、海马体和脑膜。将两个皮质半球放入含有 5 毫升 MEM EBSS 和 2X PSN 的 50 毫升锥形管中,并保持在冰上。
在组织培养罩中,使用移液管从 50 毫升锥形管中吸出培养基,将皮质组织块留在底部。用镀膜玻璃移液管加入 10 毫升预热的解离培养基,并将组织研磨 10 至 20 次。将悬浮液转移到含有小搅拌棒的 50 毫升烧杯
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