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Isolating and Infecting Primary Cortical Mixed Glia with Prions

29 Views

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06:28 min

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August 11th, 2023

DOI :

10.3791/200271-v

August 11th, 2023

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29 Views

Arielle J. D. Hay^1,2, Katriana A. Popichak^1,2, Mark D. Zabel^1,2, Julie A. Moreno^1,3

¹Prion Research Center, Colorado State University, ²Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, ³Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University

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Keywords Primary Cortical Mixed Glia

Prion Infection

Brain Tissue Isolation

Tissue Dissociation

Brain Homogenate

UV Light Exposure

Adipose Tissue Isolation

Enzymatic Digestion

From the series

Coculturing AdMSCs and Glia to Reduce Prion-Induced Inflammation

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