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Please note that all translations are automatically generated. Click here for the English version.
21 Views
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02:33 min
August 11th, 2023
DOI :
10.3791/200272-v
Transcript
用含有每毫升 10 纳克 TNF α 的培养基处理传代 24 小时的传代 AdMSC,或不含未刺激细胞的 TNF α。在无血清培养基中洗涤和孵育细胞 4 小时后,向每个插入片段中加入 25, 000 个 AdMSC,并在 37 摄氏度下孵育 24 小时。吸出两个孔中的内容物并插入,然后用 PBS 洗涤两次,在每个孔中留下一毫升 PBS。
使用镊子,一个接一个地取出插入物,然后用棉签轻轻但彻底地擦拭顶部腔室以去除未迁移的细胞。将剩余的PBS吸入,并将500微升结晶紫溶液移液到孔和
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