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02:02 min
February 24th, 2023
DOI :
10.3791/200286-v
Transcript
使用消化小鼠膝关节后获得的细胞悬浮液,并将悬浮液播种在涂有胶原蛋白的培养皿上。将培养皿在含有 5% 二氧化碳的潮湿环境中在 37 摄氏度下孵育一小时。孵育后,使用移液管收集非贴壁细胞。
用培养基清洗胶原蛋白包被的培养皿,并收集培养基。然后,向培养皿中加入新鲜培养基以培养表现出成纤维细胞样形态的贴壁细胞。在Hank的平衡盐溶液中用0.05%胰蛋白酶处理细胞,并传代亚汇合细胞。
如果需要高纯度的成纤维细胞样细胞,请进行重复传代。确保使用少于五代的细胞。从炎性关节炎组织中
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