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Please note that all translations are automatically generated. Click here for the English version.
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01:08 min
June 30th, 2023
DOI :
10.3791/200299-v
Transcript
要开始在倒置显微镜载物台上安装3D打印的实时成像插件,请小心地将插件放入大型显微镜载物台凹槽中。使用螺钉固定插件的每个角。将加热板以插头面朝下的方向滑入插件的侧开口,使加热板位于下部插件凹槽的顶部,插头端口位于载物台下方。
将插件中的凹槽圆形开口与 40 x 硅油浸没物镜对齐,并将硅油涂在物镜的中心。然后使用注射器,沿着带凹槽的圆形开口涂抹少量真空润滑脂。将盖玻片放在开口顶部,将其密封在插件上。
最后,抬起 40 倍物镜,直到它几乎不接触玻璃盖玻片的底部。
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