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Please note that all translations are automatically generated. Click here for the English version.
110 Views
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00:59 min
May 5th, 2023
DOI :
10.3791/200308-v
Transcript
首先在 14 个平板上接种大约 3, 500 个 C.elgans F1 代胚胎,让它们在 21 摄氏度下生长到年轻成虫阶段三天。使用 PBS/TX 将 C.elgans 洗涤并收集到 1.5 毫升管中。以1000G离心两分钟,吸出,并将蠕虫从一个菌株中拉入一个管中。
用 PBS/TX 重复洗涤三次。接下来,吸出上清液,在试管中留下一毫升,然后倒置混合。在三次计算三微升的C.elgans蠕虫数量后,计算蠕虫的浓度,并将相当于3000条蠕虫的体积转移到新管中进行甲醛交联。
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