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Please note that all translations are automatically generated. Click here for the English version.
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01:18 min
May 5th, 2023
DOI :
10.3791/200309-v
Transcript
首先将甲醛加入到含有蠕虫的试管中,最终浓度为1.8%,并在室温下旋转试管六分钟。然后立即将试管冷冻在液氮中,并将样品储存在80摄氏度。要继续,将甲醛交联样品在室温水浴中解冻三分钟,并在室温下旋转样品16分钟。
以1.25摩尔甘氨酸至125毫摩尔终浓度,并在室温下旋转5分钟。在此步骤之后,将样品保持在冰上或四度,并使用冰冷的缓冲液直到磁珠洗脱。将样品以 1000 g 离心 3 分钟后,用 1 毫升 PBSTX 洗涤 3 次。
然后用一毫升重悬缓冲液洗涤两次。最后一次洗涤后,留下足够的缓冲液,以确保总体积至少是颗粒体积的三倍,至少为 100 微升。
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