Name: Establishment of Murine Chronic Root Ganglion Compression Model
Uploaded: 2023-07-14T13:50:00
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establishment of murine chronic root ganglion compression model

بروتوكول Tuina الموحد لنموذج ضغط DRG للفئران

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

تسليط الضوء على المؤلف: تأثير مسكن من Tuina على نماذج الفئران مع ضغط آلام العقدة الجذر الظهرية  

تأثير مسكن التوينا على نماذج الفئران مع ضغط آلام العقدة الجذرية الظهرية

Tuina analgesia is widely used in clinical practice. Our research focuses on the mechanism of action of Tuina analgesia, and investigate how Tuina achieve its analgesic effect. Achieving standardization in Tuina is currently the most significant obstacle.The effectiveness of Tuina is influenced by several factors such as treatment, area type, intensity, and frequency. The current protocol is optimized in two aspect based on the earlier research. Firstly, the force and frequency of pushing and holding operations were standardized and controlled by force-measuring gloves.Secondly, the results of imaging and animal behavior test were integrated to assess the results of modeling.

Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

Establishment of Murine Chronic Root Ganglion Compression Model  

إنشاء نموذج ضغط العقدة الجذرية المزمنة للفئران

لإنشاء النموذج الحيواني ، ابدأ بتأمين فأر مخدر على لوح رغوي ، وربط أطرافه وقواطعه باستخدام الأربطة المطاطية. امسح المنطقة المحضرة بمسحة كحول بنسبة 75٪. بعد ذلك ، استخدم المقص لعمل شق من 2 إلى 3 سنتيمترات عبر الجلد ، واللفافة السطحية ، واللفافة العميقة طبقة تلو الأخرى.حدد موقع العمود الفقري الحرقفي العلوي الأمامي ، تليها العمليات الشائكة الثالثة والرابعة. ثبت العملية الشائكة بالملقط المسنن وارفعها للسماح للمقص بأن يكون قريبا من الجانب الأيمن من العملية الشائكة. قطع العضلات تعلق على الجانب الأيمن من عملية شائكة.ثم تشريح العضلات بصراحة تعلق على السطح الخارجي للصفيحة الفقرية حتى يتم الشعور بالمقاومة. وبالمثل ، تشريح العضلات واللفافة بصراحة على المفصل zygapophysial. استخدم مسبارا على شكل حرف L لتحديد موقع الثقبة الفقرية.بعد ذلك ، أدخل قضيبا من الفولاذ المقاوم للصدأ على شكل حرف L في الثقبة الفقرية القطنية الرابعة والخامسة. استمر في خياطة العضلات واللفافة وطبقة الجلد طبقة تلو الأخرى باستخدام 3-0 خيوط جراحية. ضع الجرذ في صندوق ثرموستاتي حتى يستيقظ ولاحظ وظيفة الطرف الخلفي الأيمن للفأر لضمان الحياة الطبيعية.

Research

JoVE Journal

Medicine

Establishment of Murine Chronic Root Ganglion Compression Model

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...