Name: Establishment of Murine Chronic Root Ganglion Compression Model
Uploaded: 2023-07-14T13:50:00
Description: Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

establishment of murine chronic root ganglion compression model

Standardisert Tuina-protokoll for Rat DRG-komprimeringsmodell

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

Forfatter Spotlight: Smertestillende effekt av Tuina på rottemodeller med kompresjon av ryggrotganglionsmerter  

Analgetisk effekt av tunina på rottemodeller med kompresjon av dorsalrotganglionsmerter

Tuina analgesia is widely used in clinical practice. Our research focuses on the mechanism of action of Tuina analgesia, and investigate how Tuina achieve its analgesic effect. Achieving standardization in Tuina is currently the most significant obstacle.The effectiveness of Tuina is influenced by several factors such as treatment, area type, intensity, and frequency. The current protocol is optimized in two aspect based on the earlier research. Firstly, the force and frequency of pushing and holding operations were standardized and controlled by force-measuring gloves.Secondly, the results of imaging and animal behavior test were integrated to assess the results of modeling.

Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

Establishment of Murine Chronic Root Ganglion Compression Model  

Etablering av Murine Chronic Root Ganglion Compression Model

For å etablere dyremodellen, start med å sikre en bedøvet rotte på et skumbrett, fest lemmer og snitt ved hjelp av gummibånd. Tørk av det forberedte området med en 75% alkoholserviett. Deretter bruker du saks til å lage et to til tre centimeter snitt gjennom huden, overfladisk fascia og dyp fascia lag for lag.Finn den fremre overlegne iliac ryggraden, etterfulgt av den tredje og fjerde spinøse prosessen. Klem den spinøse prosessen med tanntang og løft den slik at saksen kan være nær høyre side av den spinøse prosessen. Klipp muskelen festet til høyre side av den spinøse prosessen.Deretter dissekerer du sløvt muskelen festet til den ytre overflaten av vertebralplaten til motstand er følt. På samme måte dissekerer du sløvt muskelen og fascia på zygapofysialleddet. Bruk en L-formet sonde for å finne det intervertebrale foramen.Sett deretter en L-formet rustfritt stålstang inn i fjerde og femte lumbale intervertebrale foramen. Fortsett å suturere muskelen, fascia og hud lag for lag ved hjelp av 3-0 suturer. Plasser rotta i en termostatboks til den våkner og følg funksjonen til rottens høyre baklem for å sikre normalitet.

Research

JoVE Journal

Medicine

Establishment of Murine Chronic Root Ganglion Compression Model

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...