Name: Establishment of Murine Chronic Root Ganglion Compression Model
Uploaded: 2023-07-14T13:50:00
Description: Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

establishment of murine chronic root ganglion compression model

Protocolo Tuina estandarizado para el modelo de compresión DRG de ratas

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

Autor destacado: Efecto analgésico de Tuina en modelos de ratas con compresión del dolor del ganglio de la raíz dorsal  

Efecto Analgésico de Tuina en Modelos de Ratas con Compresión del Dolor del Ganglio de la Raíz Dorsal

Tuina analgesia is widely used in clinical practice. Our research focuses on the mechanism of action of Tuina analgesia, and investigate how Tuina achieve its analgesic effect. Achieving standardization in Tuina is currently the most significant obstacle.The effectiveness of Tuina is influenced by several factors such as treatment, area type, intensity, and frequency. The current protocol is optimized in two aspect based on the earlier research. Firstly, the force and frequency of pushing and holding operations were standardized and controlled by force-measuring gloves.Secondly, the results of imaging and animal behavior test were integrated to assess the results of modeling.

Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

Establishment of Murine Chronic Root Ganglion Compression Model  

Establecimiento del modelo de compresión del ganglio radicular crónico murino

Para establecer el modelo animal, comience por asegurar una rata anestesiada en una tabla de espuma, sujetando sus extremidades e incisivos con bandas elásticas. Limpie el área preparada con un hisopo con alcohol al 75%. A continuación, use unas tijeras para hacer una incisión de dos a tres centímetros a través de la piel, la fascia superficial y la fascia profunda capa por capa.Localiza la espina ilíaca anterosuperior, seguida de la tercera y cuarta apófisis espinosas. Sujete la apófisis espinosa con pinzas dentadas y levántela para permitir que las tijeras estén cerca del lado derecho de la apófisis espinosa. Corta el músculo unido al lado derecho de la apófisis espinosa.A continuación, diseccione sin rodeos el músculo unido a la superficie exterior de la placa vertebral hasta que se sienta resistencia. Del mismo modo, diseccione sin rodeos el músculo y la fascia en la articulación zigapofisiaria. Use una sonda en forma de L para ubicar el foramen intervertebral.A continuación, inserte una varilla de acero inoxidable en forma de L en el cuarto y quinto agujero intervertebral lumbar. Proceda a suturar el músculo, la fascia y la piel capa por capa utilizando suturas 3-0. Coloque a la rata en una caja termostática hasta que se despierte y observe la función de la extremidad trasera derecha de la rata para garantizar la normalidad.

Research

JoVE Journal

Medicine

Establishment of Murine Chronic Root Ganglion Compression Model

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...