Uploaded: 2023-07-21T14:55:00
Description: Watch this Scientific Journal Video about Determining the End-Systolic and End-Diastolic Pressure-Volume Relationships in a Murine Heart Failure Model and Transesophageal Pacing at JoVE.com

determining the end-systolic and end-diastolic pressure-volume relationships in a murine heart failure model and transesophageal pacing

A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models

Watch this Scientific Journal Video about Determining the End-Systolic and End-Diastolic Pressure-Volume Relationships in a Murine Heart Failure Model and Transesophageal Pacing at JoVE.com

确定小鼠心力衰竭模型和经食管起搏中的收缩末期和舒张末期压力-容积关系

首先，将麻醉的动物放置在手术区域，确保手术部位得到充分的剃须和消毒。接下来，通过标准外科手术正确放置电导导管。要记录和分析基线压力量或 PV 回路，请在实验室图表软件中选择基线参数，然后单击立即分析按钮。选择基线选项。打开血流动力学表并选择平均值数据。通过使用镊子压缩 IVC 进行下腔静脉或 IVC 闭塞。在软件中，手动选择左心室压和容积都降低的IVC闭塞点。然后，通过选择闭塞选项，然后单击“立即分析”按钮，记录至少 20 个心动周期的 PV 循环，而 IVC 保持闭塞状态。要进行经食管起搏，请将 2 法式四极电极导管插入食管。将导管连接到脉冲刺激器，然后继续确定心房捕获阈值。为了将心率减慢到每分钟 400 次以下，腹膜内注射每公斤 20 毫克伊伐布雷定。稳定后，获取 20 个连续心动周期的 PV 环，同时以每分钟 400 至 700 次的不同速率起搏心脏。基线PV环数据显示，对照组和HFpEF小鼠之间的舒张期参数（如弛豫时间常数，最小压力变化率和舒张末期压力容积关系或EDPVR）没有显着差异。然而，HFpEF小鼠表现出较高的血压和动脉弹性蛋白，在心室收缩期间表现出典型的山形PV环。该PV环路与心室肌肉直接接触压力传感器引起的尖峰不同。值得注意的是，使用心房起搏，可以清楚地区分对照小鼠和HFpEF小鼠之间的舒张功能。在对照组中，弛豫时间常数和EDPVR均随着起搏率的增加而改善。而在HFpEF组中，随着心率随着心房起搏的增加，弛豫时间常数和EDPVR均恶化。

Research

JoVE Journal

Biology

Determining the End-Systolic and End-Diastolic Pressure-Volume Relationships in a Murine Heart Failure Model and Transesophageal Pacing

Exploring HR-Dependent Cardiac Performance Using an Atrial-Pacing Protocol

Investigating HR-Dependent Cardiac Function in Mouse Models Through a Novel Atrial-Pacing Approach (Video) | JoVE

用于评估小鼠心力衰竭模型心率依赖性舒张功能的起搏控制程序

Heart failure with preserved ejection fraction (HFpEF) is a condition characterized by diastolic dysfunction and exercise intolerance. While ...

科研

生物学

Instrument and Animal Preparation for Catheterization in a Murine Heart Failure Model

Instrument and Animal Preparation for Catheterization in a Murine Heart Failure Model (Video) | JoVE 

小鼠心力衰竭模型导管插入术的器械和动物准备

Surgical Procedure for Left Ventricular Catheterization (Open Chest Approach) in a Murine Heart Failure Model

Surgical Procedure for Left Ventricular Catheterization (Open Chest Approach) in a Murine Heart Failure Model (Video) | JoVE 

小鼠心力衰竭模型中左心室导管插入术（开胸入路）的外科手术

Saline Calibration and Aortic Flow Calibration in a Murine Heart Failure Model

Saline Calibration and Aortic Flow Calibration in a Murine Heart Failure Model (Video) | JoVE 

鼠心力衰竭模型中的盐水校准和主动脉血流校准