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Sample Preparation and Fluorescence Measurement of Stained Transfected HEK293T Cells (Video) | JoVE

DOI :

10.3791/200360-v

•

June 2nd, 2023

June 2nd, 2023

•
101 Views
1Department of Life Sciences and National Institute for Biotechnology in the Negev, Ben-Gurion University, 2Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University

Tags

Keywords Sample Preparation
Fluorescence Measurement
Transfected HEK293T Cells
Microscope
Light Source
Camera
Suction System
Ringer s Solution
Ringer s Zinc Solution
Pyrithione
Perfusion Chamber
Sealing Silicone
Coverslip
Ringer s Washing Solution
Perfusion Rate
Imaging Software
MCherry
ROIs
Background ROI
GFP
Perfect Focus System
PFS

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