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Please note that all translations are automatically generated. Click here for the English version.
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03:36 min
July 7th, 2023
DOI :
10.3791/200375-v
Transcript
首先,用70%乙醇彻底喷洒处死的10周龄C57黑色6J雄性小鼠。使用镊子从后肢上去除皮肤,并解剖股骨、胫骨和腓骨周围的所有肢体肌肉。将收获的肢体肌肉放入含有一毫升肌肉隔离缓冲液的两毫升管中。
并用剪刀将收获的肌肉切碎。将切碎的肌肉悬液转移到含有 18 毫升肌肉隔离缓冲液的 50 毫升管中。将试管盖紧,用实验室薄膜密封,水平置于摇动的水浴中。
30 分钟后,加入 5 毫克 I 型胶原酶,并再保持试管 30 分钟。将肌肉悬液上下移液10次后,离心并弃去上清液,在试管中留
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