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Please note that all translations are automatically generated. Click here for the English version.
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03:26 min
August 11th, 2023
DOI :
10.3791/200380-v
Transcript
麻醉小鼠后,轻轻捏住小鼠的一只爪子,检查小鼠是否被充分麻醉。施用1%托吡卡胺滴眼液以散瞳小鼠的眼睛并等待30秒。然后,为了减少眼球运动和眨眼,将0.5%盐酸丙丙卡因滴在双眼上,然后涂上润滑剂眼凝胶滴剂,并将鼠标放在加热水垫上。
制备含有等体积吲哚菁绿或ICG和荧光素染料的染料混合物。通过腹膜内注射将250微升混合物注射到小鼠后腿附近的左下象限,使其平行于皮肤定位以防止器官穿孔。小心地缩回柱塞,确保没有血液进入注射器盖。
以稳定的速度逐渐注入染料。接下来,将鼠标放在成
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