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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing

119 Views

•

05:45 min

•

July 7th, 2023

DOI :

10.3791/200389-v

July 7th, 2023

•
119 Views
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

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Poly A RNA Purification
Bisulfite Conversion
Next generation Sequencing
Total RNA
Oligo dT Beads
Lysis Buffer
Wash Buffer
Tris HCl
RNA Elution
Magnetic Separation
RNA Sample Preparation
RNA Enrichment

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Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping
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