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Please note that all translations are automatically generated. Click here for the English version.
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01:47 min
August 4th, 2023
DOI :
10.3791/200400-v
Transcript
首先,将 200 微升细菌接种物添加到无菌聚苯乙烯 96 孔板的每个孔中。然后在最外层的孔中加入 200 微升去离子水,以防止内孔变干。将 96 孔板在 25 摄氏度下孵育 24 小时。
孵育后,使用移液管弃去每个孔中的上清液。小心地向每个孔中加入 300 微升 PBS,然后使用移液器再次除去 PBS。向每个孔中加入 200 微升 1% 结晶紫溶液,然后在室温下孵育板 30 分钟。
然后在每个孔中分配 200 微升无水乙醇后,将板在室温下放置 15 分钟。通过反复上
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