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Please note that all translations are automatically generated. Click here for the English version.
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01:44 min
September 1st, 2023
DOI :
10.3791/200434-v
Transcript
首先,将硼硅酸盐玻璃毛细管连接到手动立式微量移液器拉拔器中的橡胶缓冲夹上。将微量移液器拉拔器的加热温度设置为950摄氏度,并进行预拉,以获得更薄、更软的玻璃毛细管。然后将加热温度设置为790摄氏度,并进行二次拉动以产生最终的微量移液器。
使用微量移液器研磨机,小心地将吸头开口锐化至外径 11 微米。然后将微量移液器放入玻璃罐内的海绵夹紧垫中。将受精鸡蛋在37摄氏度的加湿摇摆箱中孵育至发育阶段18。
用 70% 乙醇彻底擦拭工作区域。发育后,从培养箱中取出鸡蛋。用70
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