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Please note that all translations are automatically generated. Click here for the English version.
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02:26 min
September 1st, 2023
DOI :
10.3791/200435-v
Transcript
首先,在冰上解冻RCAS(A)逆转录病毒储备液。为避免堵塞微量移液器,将溶液以 10, 000 G 在 4 摄氏度下离心 10 秒,然后将上清液转移到新管中,同时将溶液保持在冰上。将拉伸的实验室石蜡膜放在 35 x 10 毫米的细胞培养皿上,并在石蜡膜中加入一微升无菌 PBS。
将准备好的玻璃微量移液器连接到自动 Pico 进样器。按下填充模式将PBS填充到微量移液器中,然后按注射模式测试分配的一微升液体。将第二片拉伸的实验室封口膜放在新的细胞培养皿上,并在薄膜中加入一微升快速
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