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02:10 min
June 23rd, 2023
DOI :
10.3791/200448-v
Transcript
将幼虫收集容器完全浸入培养罐中。接下来,将珊瑚群浸入幼虫容器中,确保两者都保持在水下。然后将幼虫收集容器的手柄钩在吊杆上。
第二天早上,从吊杆上解开钓鱼线,小心地从幼虫收集容器中取出一个菌落。立即将菌落放回培养罐中。接下来,从水箱中取出幼虫收集容器并将其放在量壶的顶部。
用一根手指在盖子上施加适度的压力,然后拧下。将量壶中的水倒入碗中。手动计算碗中的幼虫,使用三毫升移液器将它们转移到 50 毫升的试管中。
该方法能够评估不同温度和摄食处理下Pocillo
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