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Please note that all translations are automatically generated. Click here for the English version.
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03:54 min
October 6th, 2023
DOI :
10.3791/200455-v
Transcript
首先将麻醉的小鼠放在加热的手术架上的右侧,以暴露其左腹部。用纸胶带固定鼠标的前端和后肢,确保脾脏可见。接下来,通过大量涂抹消毒剂对手术部位的皮肤进行消毒。
通过捏脚趾来确认小鼠完全麻醉。使用镊子,抬起左上腹部的皮肤,用卡斯特罗维霍剪刀在皮肤和肌肉层上做一个10毫米的圆形切口。找到附着在脾脏上的胰腺,以确定十字绣的放置方向。
使用 5/0 真丝缝合线将第一针缝入肌肉层的确定位置。用三到五节固定末端。继续直接缝合切口。
然后剪掉并留下大约五厘米的尾巴。重复缝
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