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Please note that all translations are automatically generated. Click here for the English version.
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01:51 min
October 20th, 2023
DOI :
10.3791/200457-v
Transcript
首先,拿一瓶苍蝇,每七天将它们转移到一个新瓶子中,与它们的生长周期同步。在转移三天后收集从初始瓶子孵化的下一代苍蝇,并将它们放入新瓶子中。接下来,将玻璃管放入一个大烧杯中。
倒入双蒸水,煮沸20分钟。然后取出并捆扎玻璃管。用双蒸水冲洗三到五次,然后将玻璃管放入烤箱中晾干。
使用移液管,将 4 毫升样品缓冲液和利血平加入 10 毫升小烧杯中。对于阴性对照组,加入二甲基亚砜至浓度达到0.2%小心地将适当长度的玻璃管插入小烧杯中,以利于培养基的流动。等待培养基完全凝固。<
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