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Please note that all translations are automatically generated. Click here for the English version.
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06:10 min
July 28th, 2023
DOI :
10.3791/200464-v
* These authors contributed equally
Transcript
首先将单个细菌菌落接种到 500 毫升锥形瓶中的 200 毫升灭菌的 Luria 肉汤中。将细菌在设置为37度的摇床培养箱中培养过夜。培养14小时后,检查细菌在600纳米处的光密度。
将细菌分装到 50 毫升锥形管中,并将它们储存在 4 摄氏度以备将来使用。使用血清移液管,将 50 毫升细菌培养物转移到新的 250 毫升锥形瓶中,标记试管以进行活体或模拟处理对照。洗涤前使用血清移液管将 50 毫升细菌转移到 50 毫升锥形管中,用于模拟处理的对照。
在化学罩中,向细
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