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Please note that all translations are automatically generated. Click here for the English version.
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03:15 min
August 25th, 2023
DOI :
10.3791/200477-v
Transcript
在零日,将 1000 万个 293T 细胞接种在 30 毫升不含抗生素的 DMEM 中,其中含有 10% FBS,放入 15 厘米培养皿中。总共准备10道这样的菜肴。在第二天,即第一天,确认细胞汇合度达到 80% 并准备好进行转染。
然后在50毫升锥形管中依次加入600微升每微升0.5微克的包装质粒混合物和60微升质粒条形码文库。在单独的 15 毫升锥形管中,通过涡旋混合 900 微升转染试剂和 12 毫升 DMEM。将 12.9 毫升这种混合物加入 DNA 混合物中,然后轻弹
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