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Please note that all translations are automatically generated. Click here for the English version.
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02:59 min
February 9th, 2024
DOI :
10.3791/200484-v
Transcript
首先将 0.0005 克 NBT 溶解在 10 微升 DMSO 中。涡旋 15 分钟后,加入 90 微升含钙和镁的 Hank's 平衡盐溶液或 HBSS 以及 1 微升测试物质。再次,涡旋管最多两分钟。
为了进行NBT载玻片测试,轻轻地混合并转移两微升先前制备的活化多形核中性粒细胞到干净的载玻片上。将载玻片在 37 摄氏度的加湿室中孵育 20 分钟。接下来,在细胞上加入一微升NBT工作溶液。
再次孵育 20 分钟,避光。干燥载玻片后,通过在每个孔中滴一滴甲醇一分钟来
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