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01:22 min
February 9th, 2024
DOI :
10.3791/200487-v
Transcript
轻轻地混合并转移四微升先前制备的活化多形核中性粒细胞系统,以评估到干净载玻片的两个孔中。将载玻片在 37 摄氏度的加湿室中孵育 30 分钟。将一微升 DNase I 加入其中一个孔中,并在 37 摄氏度下孵育 20 分钟。
用快速全景染色后,在显微镜下评估。对全景染色的中性粒细胞进行定性分析表明,对照组和fMLP组没有显示出任何NET释放的迹象,而PMA诱导的NET样结构形成的活化被DNase 1处理降解。抗菌肽对中性粒细胞的影响表明,这种刺激可能能够引起NET释放,因为载玻片
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