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Please note that all translations are automatically generated. Click here for the English version.
94 Views
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00:58 min
November 10th, 2023
DOI :
10.3791/200492-v
Transcript
首先,使用带有过滤网的提取袋收获六到八周龄的马铃薯病毒感染的彭特兰马铃薯植株。每克组织加入 4 毫升磷酸盐缓冲液,补充 DIECA。然后用手动均质器将植物均质化一到两分钟。
接下来,在三到四个星期大的健康马铃薯植株的前三片完全发育的叶子上轻轻涂上碳化硅粉。用准备好的病毒接种物轻轻擦拭叶子。10分钟后,用自来水将叶子冲洗干净。
最后,将植物转移到生长室中,让它们在28摄氏度的受控环境条件下生长三天。
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