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Please note that all translations are automatically generated. Click here for the English version.
20 Views
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01:21 min
August 25th, 2023
DOI :
10.3791/200506-v
Transcript
首先将四只成虫放在接种有大肠杆菌OP50的线虫生长培养基或NGM琼脂平板上。在23摄氏度的温度下,在NGM板上培养第一代蠕虫,使其挨饿7天。接下来,将少量狗粮琼脂或DFA培养基转移到接种有大肠杆菌OP50的新NGM板的中心。
对于光遗传学实验,在蠕虫接种之前将 40 微升全反式视网膜醛倒入 DFA 上。接下来,0.5毫克的狗粮在DFA培养基上,距离板盖约两毫米。用紫外线照射 NGM 板 15 分钟,避免污染。
使用消毒水从NGM板中收集饥饿的蠕虫。然后将收集的蠕虫接
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