Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
19 Views
•
01:12 min
August 25th, 2023
DOI :
10.3791/200507-v
Transcript
首先将不含大肠杆菌和DFA的新NGM板放在微距变焦显微镜载物台上的铝板上。使用帕尔贴温度控制器将板底保持在 23 摄氏度温度至少五分钟。接下来,用线虫爬到的盘子的盖子替换盘子。
将板底温度提高到 26 摄氏度以改变内部湿度。现在,使用显微镜相机以每秒 20 帧的速度捕获板盖内表面的图像,将 ZX899 蜗杆保持在 DFA 上,在照明前保持此状态 5 分钟。现在照亮连接到培养皿盖的ZX899蠕虫,保持在23摄氏度。
以每秒 20 帧的速度捕获内板表面的图像。湿度的增加
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved