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Please note that all translations are automatically generated. Click here for the English version.
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01:51 min
August 18th, 2023
DOI :
10.3791/200510-v
Transcript
首先,将预先浸泡的竹子播种到土壤和蛭石的基质中发芽。对于农杆菌感染,小心地从基质上取出15天大的幼苗,土壤附着根。用锡纸包裹幼苗,以保持水分并防止土壤脱落。
将包裹好的幼苗转移到湿度较高、光照度较低的植物生长室中两个小时。接下来,使用注射器中的锋利针头,将卷曲的未成熟叶子的上部缠绕一次或两次。迅速将叶子的受伤部分浸入农杆菌悬浮液中。
然后立即将幼苗转移到压力为 25 至 27 毫米汞柱的真空室中两分钟。吸尘后,小心地打开幼苗并将其重新种植到基质中。让幼苗在高湿度的昏
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