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03:33 min
August 18th, 2023
DOI :
10.3791/200511-v
Transcript
首先从野生型和农杆菌感染的竹叶中提取基因组DNA。使用以下PCR条件扩增来自野生型和农杆菌感染的竹叶中含有靶基因靶位点的基因组DNA。PCR后,通过制备含有1μL age-1,1μgPCR产物和5μL 10X缓冲液的反应混合物进行核酸内切酶消化。
然后,加水至最终体积为 50 微升。将消化混合物在 37 摄氏度下孵育一小时。使用凝胶电泳,分析消化的DNA片段的比例。
比较野生型和农杆菌感染样本的消化片段,以评估基因编辑效率。将竹苗暴露在高光强度条件下,以增加吸收光量
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