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Please note that all translations are automatically generated. Click here for the English version.
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04:47 min
October 6th, 2023
DOI :
10.3791/200531-v
Transcript
首先,放置麻醉的 C57 Black/6 JRj 鼠标。仰卧在加热的手术台上。确认对脚趾捏合没有反射反应。
用70%乙醇喷洒鼠标,以防止头发粘在剪刀上。用剪刀在底部做一个腹部切口,从两侧向上切到肋骨,露出腹腔。用棉签将肠道向右移动,露出门静脉。
使用弯曲的镊子,在门静脉下放置两个结扎带,并松散地绑住每个结扎带。接下来,将一根 0.7 毫米的导管插入门静脉。穿孔时,从导管上取下针头,引导导管穿过静脉,直到导管尖端靠近肝脏。
然后,收紧结扎。观察肝脏。设置滚
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