electroacupuncture of multiple rodents in a 3d printed fixator

Utilisation d’un support imprimé en 3D pour l’électroacupuncture en vrac chez les petits animaux

Electroacupuncture (EA) therapy is a unique method in which acupuncture needles are inserted into the scalp and connected to an electro-machine to stimulate specific points1. Unlike manual stimulation, EA allows better control of stimulation by stabilizing specific frequencies and waveforms to achieve optimal therapeutic effects2. According to a survey, 81.2% of medical institutions in China use EA or manual acupuncture to treat cerebral palsy, neuralgia, facial palsy, and other conditions. Despite its popularity, the specific mechanism of efficacy of EA is still unknown, which has hindered its promotion and application in the rehabilitation treatment of neurological diseases1,2,3. Further research is needed to fully understand the mechanism of EA&#39;s effectiveness and to promote its use in the rehabilitation treatment of neurological diseases.
As the influence of acupuncture expands worldwide, many studies have already investigated the mechanisms of EA performed on rodent models, such as rat or mouse models1,2,3. Several issues are often encountered in EA&#39;s rodent experiments. The first is how to immobilize rodents without anesthesia, as acupuncture performed on awake subjects is more reflective of clinical practice. In addition, some experiments require animals to be awake to observe treatment effects2,3. Another challenge is accurately locating the acupoints in mice or rats that correspond to those in humans. The precise localization of acupoints in experimental rodents is currently being studied by many scholars4,5. In this protocol, MS6 and ST25 were selected, which have been clearly defined in rodents by the transformation of human anatomical localization. MS6 is commonly used for the treatment of some brain diseases, such as Parkinson&#39;s disease6. ST25 is usually used to treat gastrointestinal problems, such as diarrhea7. These two acupoints were chosen primarily to demonstrate how rodents can be effectively immobilized in both supine and prone positions. Moreover, these acupoints have been extensively studied and offer significant insights for research purposes6,7.
The previous method of immobilizing a single rat for experiments is not only time-consuming but also difficult to handle by a single person8. Additionally, due to the non-cooperation of animals, the success rate is relatively low in practice. Therefore, there is a critical need to create an easily established animal model with stable characteristics to improve experiment efficiency. In this article, a 3D printed holder for small animals was introduced that could easily immobilize multiple rodents, leading to motor restriction. The aim of this paper is to administer EA treatments to a batch of young rats or mice, focusing on the strategies for bulk restriction of mice, identification, and stimulation of MS6 and ST25 acupoints.

Pleins feux sur l’auteur : Utilisation d’un support imprimé en 3D pour une opération d’électroacupuncture multiple simultanée

Opération d’électroacupuncture en vrac pour souris ou jeunes rats

In this video, we introduce an efficient way of using electroacupuncture on multiple awake mice or rats. In vesical research, animal is urinating, but they are not cooperative, and acupuncture only is time consume. Another issue is about the anesthesia, which is not clinical applicable of acupuncture.To address these issues, we demonstrate efficient method of performing electroacupuncture on multiple awake mice or rats by using 3D printed holder. Our protocol demonstrates a more efficiency and safer way to hold multiple animals simultaneously, which unlike other method that imply immobilization, this method not only saves time, but also improve experimental success rates. Additionally, device is designed to be easy to handle, which could be operated by one person.The ProTech and device are beneficial for the promotion of experimental scientific research in electroacupuncture.

Électroacupuncture de plusieurs rongeurs dans un fixateur imprimé en 3D

Pour commencer, divisez une corde de deux mètres de long en six segments égaux. Utilisez le mécanisme de boucle à ressort pour attacher les extrémités des cordes, ce qui permet d’ajuster facilement la taille des boucles. Enfilez chacune des six cordes dans les trous désignés dans le fixateur.Placez ensuite la tête du rongeur dans le trou en forme de U du fixateur. Attachez soigneusement les boucles autour du cou du rongeur et guidez doucement son corps à travers deux des boucles de corde préparées. Fixez ensuite les boucles autour de l’abdomen du rongeur, en vous assurant qu’il est suffisamment serré sans causer de détresse respiratoire.Immobiliser de la même manière les deux autres rongeurs. Trouvez les points d’acupuncture par emplacement anatomique et confirmez la profondeur d’insertion au niveau du point d’acupuncture. Tournez lentement et insérez les aiguilles stériles jetables bilatéralement et sous-cutanéement dans MS6 ou ST25.Ensuite, connectez un côté du câble de connexion à l’instrument d’électroacupuncture. Connectez l’autre côté du câble de connexion avec les clips positifs et négatifs aux tiges d’aiguille d’acupuncture à gauche et à droite MS6. Allumez l’instrument d’électroacupuncture.Faites pivoter la fonction ou choisissez le bouton de commutation pour régler la fréquence et le mode de fonction. Réglez la fréquence sur 10 Hertz et choisissez un mode d’onde continue. Tournez le bouton Ajuster l’intensité pour régler l’intensité à un à deux milliampères pendant cinq minutes.Le fixateur imprimé en 3D a permis l’immobilisation ferme simultanée de trois souris en position couchée et couchée sur le ventre sans blessure. Les points d’acupuncture MS6 et ST25 ont été localisés avec succès et piqués chez les rongeurs. Le temps moyen d’immobilisation de trois rats dans les deux positions était compris entre 72 et 77 secondes.

Research

JoVE Journal

Biology

Electroacupuncture of Multiple Rodents in a 3D Printed Fixator

Bulk Electroacupuncture Operation for Mice or Young Rats

Electroacupuncture (EA) is widely used to treat various health conditions. However, the underlying mechanism of EA treatment remains unclear, hindering ...

Recherche

Biologie