electroacupuncture of multiple rodents in a 3d printed fixator

Utilizzo di un supporto stampato in 3D per l'elettroagopuntura di massa nei piccoli animali

Electroacupuncture (EA) therapy is a unique method in which acupuncture needles are inserted into the scalp and connected to an electro-machine to stimulate specific points1. Unlike manual stimulation, EA allows better control of stimulation by stabilizing specific frequencies and waveforms to achieve optimal therapeutic effects2. According to a survey, 81.2% of medical institutions in China use EA or manual acupuncture to treat cerebral palsy, neuralgia, facial palsy, and other conditions. Despite its popularity, the specific mechanism of efficacy of EA is still unknown, which has hindered its promotion and application in the rehabilitation treatment of neurological diseases1,2,3. Further research is needed to fully understand the mechanism of EA&#39;s effectiveness and to promote its use in the rehabilitation treatment of neurological diseases.
As the influence of acupuncture expands worldwide, many studies have already investigated the mechanisms of EA performed on rodent models, such as rat or mouse models1,2,3. Several issues are often encountered in EA&#39;s rodent experiments. The first is how to immobilize rodents without anesthesia, as acupuncture performed on awake subjects is more reflective of clinical practice. In addition, some experiments require animals to be awake to observe treatment effects2,3. Another challenge is accurately locating the acupoints in mice or rats that correspond to those in humans. The precise localization of acupoints in experimental rodents is currently being studied by many scholars4,5. In this protocol, MS6 and ST25 were selected, which have been clearly defined in rodents by the transformation of human anatomical localization. MS6 is commonly used for the treatment of some brain diseases, such as Parkinson&#39;s disease6. ST25 is usually used to treat gastrointestinal problems, such as diarrhea7. These two acupoints were chosen primarily to demonstrate how rodents can be effectively immobilized in both supine and prone positions. Moreover, these acupoints have been extensively studied and offer significant insights for research purposes6,7.
The previous method of immobilizing a single rat for experiments is not only time-consuming but also difficult to handle by a single person8. Additionally, due to the non-cooperation of animals, the success rate is relatively low in practice. Therefore, there is a critical need to create an easily established animal model with stable characteristics to improve experiment efficiency. In this article, a 3D printed holder for small animals was introduced that could easily immobilize multiple rodents, leading to motor restriction. The aim of this paper is to administer EA treatments to a batch of young rats or mice, focusing on the strategies for bulk restriction of mice, identification, and stimulation of MS6 and ST25 acupoints.

Autore in primo piano: Utilizzo di un supporto stampato in 3D per l'operazione di elettroagopuntura multipla simultanea

Operazione di elettroagopuntura di massa per topi o ratti giovani

In this video, we introduce an efficient way of using electroacupuncture on multiple awake mice or rats. In vesical research, animal is urinating, but they are not cooperative, and acupuncture only is time consume. Another issue is about the anesthesia, which is not clinical applicable of acupuncture.To address these issues, we demonstrate efficient method of performing electroacupuncture on multiple awake mice or rats by using 3D printed holder. Our protocol demonstrates a more efficiency and safer way to hold multiple animals simultaneously, which unlike other method that imply immobilization, this method not only saves time, but also improve experimental success rates. Additionally, device is designed to be easy to handle, which could be operated by one person.The ProTech and device are beneficial for the promotion of experimental scientific research in electroacupuncture.

Elettroagopuntura di più roditori in un fissatore stampato in 3D

Per iniziare, dividi una corda lunga due metri in sei segmenti uguali. Utilizzare il meccanismo della fibbia a molla per fissare le estremità delle corde, consentendo una facile regolazione delle dimensioni dell'anello. Infilare ciascuna delle sei corde attraverso i fori designati nel fissatore.Quindi posizionare la testa del roditore all'interno del foro a forma di U nel fissatore. Fissa con cura gli anelli intorno al collo del roditore e guida delicatamente il suo corpo attraverso due degli anelli di corda preparati. Quindi allacciare gli anelli intorno all'addome del roditore, assicurandosi che sia abbastanza stretto senza causare difficoltà respiratorie.Allo stesso modo immobilizza gli altri due roditori. Trovare i punti di agopuntura in base alla posizione anatomica e confermare la profondità di inserimento in corrispondenza del punto di agopuntura. Ruotare lentamente e inserire gli aghi sterili monouso bilateralmente e sottocutaneamente nell'MS6 o nell'ST25.Quindi, collegare un lato del cavo di collegamento allo strumento di elettroagopuntura. Collegare l'altro lato del cavo di collegamento con le clip positive e negative ai gambi dell'ago per agopuntura a sinistra e a destra MS6. Accendere lo strumento di elettroagopuntura.Ruotare la funzione o scegliere il pulsante di commutazione per impostare la frequenza e la modalità funzione. Impostare la frequenza su 10 Hertz e scegliere una modalità a onda continua. Ruotare il pulsante Regola intensità per impostare l'intensità a uno o due milliampere per cinque minuti.Il fissatore stampato in 3D ha permesso l'immobilizzazione simultanea di tre topi sia in posizione supina che prona senza lesioni. I punti di agopuntura MS6 e ST25 sono stati localizzati con successo e agugliati nei roditori. Il tempo medio per l'immobilizzazione di tre ratti in entrambe le posizioni è stato compreso tra 72 e 77 secondi.

Research

JoVE Journal

Biology

Electroacupuncture of Multiple Rodents in a 3D Printed Fixator

Bulk Electroacupuncture Operation for Mice or Young Rats

Electroacupuncture (EA) is widely used to treat various health conditions. However, the underlying mechanism of EA treatment remains unclear, hindering ...

Ricerca

Biologia