electroacupuncture of multiple rodents in a 3d printed fixator

Küçük hayvanlarda toplu elektroakupunktur için 3D baskılı bir tutucu kullanma

Electroacupuncture (EA) therapy is a unique method in which acupuncture needles are inserted into the scalp and connected to an electro-machine to stimulate specific points1. Unlike manual stimulation, EA allows better control of stimulation by stabilizing specific frequencies and waveforms to achieve optimal therapeutic effects2. According to a survey, 81.2% of medical institutions in China use EA or manual acupuncture to treat cerebral palsy, neuralgia, facial palsy, and other conditions. Despite its popularity, the specific mechanism of efficacy of EA is still unknown, which has hindered its promotion and application in the rehabilitation treatment of neurological diseases1,2,3. Further research is needed to fully understand the mechanism of EA&#39;s effectiveness and to promote its use in the rehabilitation treatment of neurological diseases.
As the influence of acupuncture expands worldwide, many studies have already investigated the mechanisms of EA performed on rodent models, such as rat or mouse models1,2,3. Several issues are often encountered in EA&#39;s rodent experiments. The first is how to immobilize rodents without anesthesia, as acupuncture performed on awake subjects is more reflective of clinical practice. In addition, some experiments require animals to be awake to observe treatment effects2,3. Another challenge is accurately locating the acupoints in mice or rats that correspond to those in humans. The precise localization of acupoints in experimental rodents is currently being studied by many scholars4,5. In this protocol, MS6 and ST25 were selected, which have been clearly defined in rodents by the transformation of human anatomical localization. MS6 is commonly used for the treatment of some brain diseases, such as Parkinson&#39;s disease6. ST25 is usually used to treat gastrointestinal problems, such as diarrhea7. These two acupoints were chosen primarily to demonstrate how rodents can be effectively immobilized in both supine and prone positions. Moreover, these acupoints have been extensively studied and offer significant insights for research purposes6,7.
The previous method of immobilizing a single rat for experiments is not only time-consuming but also difficult to handle by a single person8. Additionally, due to the non-cooperation of animals, the success rate is relatively low in practice. Therefore, there is a critical need to create an easily established animal model with stable characteristics to improve experiment efficiency. In this article, a 3D printed holder for small animals was introduced that could easily immobilize multiple rodents, leading to motor restriction. The aim of this paper is to administer EA treatments to a batch of young rats or mice, focusing on the strategies for bulk restriction of mice, identification, and stimulation of MS6 and ST25 acupoints.

Yazar Spot Işığı: Eşzamanlı Çoklu Elektroakupunktur İşlemi için 3D Baskılı Bir Tutucu Kullanma

Fareler veya Genç Sıçanlar için Toplu Elektroakupunktur Operasyonu

In this video, we introduce an efficient way of using electroacupuncture on multiple awake mice or rats. In vesical research, animal is urinating, but they are not cooperative, and acupuncture only is time consume. Another issue is about the anesthesia, which is not clinical applicable of acupuncture.To address these issues, we demonstrate efficient method of performing electroacupuncture on multiple awake mice or rats by using 3D printed holder. Our protocol demonstrates a more efficiency and safer way to hold multiple animals simultaneously, which unlike other method that imply immobilization, this method not only saves time, but also improve experimental success rates. Additionally, device is designed to be easy to handle, which could be operated by one person.The ProTech and device are beneficial for the promotion of experimental scientific research in electroacupuncture.

3D baskılı bir fiksatörde birden fazla kemirgenin elektroakupunkturu

Başlamak için, iki metre uzunluğundaki bir ipi altı eşit parçaya bölün. Halatların uçlarını sabitlemek için yaylı toka mekanizmasını kullanın, bu da ilmek boyutlarının kolayca ayarlanmasını sağlar. Altı ipin her birini fiksatörde belirtilen deliklerden geçirin.Daha sonra kemirgenin kafasını fiksatördeki U şeklindeki deliğin içine yerleştirin. İlmekleri kemirgenin boynuna dikkatlice sabitleyin ve vücudunu hazırlanan ip ilmeklerinden ikisinden nazikçe geçirin. Ardından, ilmekleri kemirgenin karnının etrafına sabitleyin ve solunum sıkıntısına neden olmadan yeterince sıkı olduğundan emin olun.Benzer şekilde diğer iki kemirgeni de hareketsiz hale getirin. Akupunktur noktalarını anatomik konuma göre bulun ve akupunktur noktasındaki yerleştirme derinliğini onaylayın. Tek kullanımlık steril iğneleri yavaşça bükün ve iki taraflı ve deri altından MS6 veya ST25'e yerleştirin.Ardından, bağlantı kablosunun bir tarafını elektroakupunktur cihazına bağlayın. Bağlantı kablosunun diğer tarafını pozitif ve negatif klipslerle sol ve sağ MS6'daki akupunktur iğnesi saplarına bağlayın. Elektroakupunktur aletini açın.Frekansı ve işlev modunu ayarlamak için işlevi döndürün veya anahtar düğmesini seçin. Frekansı 10 Hertz'e ayarlayın ve sürekli bir dalga modu seçin. Yoğunluğu beş dakika süren bir ila iki miliampere ayarlamak için Yoğunluğu Ayarla düğmesini çevirin.3D baskılı fiksatör, üç farenin hem sırtüstü hem de yüzüstü pozisyonlarda yaralanmadan aynı anda sağlam bir şekilde hareketsiz hale getirilmesine izin verdi. MS6 ve ST25 akupunktur noktaları başarılı bir şekilde yerleştirildi ve kemirgenlerde iğnelendi. Her iki pozisyonda üç sıçanın immobilizasyonu için ortalama süre 72 ila 77 saniye arasındaydı.

Research

JoVE Journal

Biology

Electroacupuncture of Multiple Rodents in a 3D Printed Fixator

Bulk Electroacupuncture Operation for Mice or Young Rats

Electroacupuncture (EA) is widely used to treat various health conditions. However, the underlying mechanism of EA treatment remains unclear, hindering ...

Biyoloji