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Please note that all translations are automatically generated. Click here for the English version.
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02:06 min
September 15th, 2023
DOI :
10.3791/200569-v
Transcript
首先,在去离子水中轻轻冲洗接种有真菌孢子的玉米叶鞘。将护套放在干净的玻璃显微镜载玻片上,近轴表面朝下,背轴表面朝上。使用单刃剃须刀片,从鞘末端修剪约一厘米,并去除中肋两侧的大部分层组织。
将剃须刀片握成 90 度角,从远轴中肋剃除组织,露出接种点上方近轴表面上的表皮层。小心地将护套部分从一个边缘提起,并将其转移到新的载玻片上,使近轴表面朝向最靠近物镜的最上层。将 60 微升去离子水涂在切片上,并在不引入气泡的情况下放置玻璃盖玻片。
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