Name: Establishment of a Rat Model of Skeletal Muscle Injury
Uploaded: 2023-08-25T14:30:00
Description: Watch this Scientific Journal Video about Establishment of a Rat Model of Skeletal Muscle Injury at JoVE.com

establishment of a rat model of skeletal muscle injury

Un massaggiatore basato sulla manipolazione del rullo di Ding per trattare le lesioni muscolari in un modello di ratto

Muscle injuries are common traumatic injuries in clinical and daily life, caused by external blows (contusions) or chronic overstrain of muscle fibers (strains), etc., resulting in muscle dysfunction and pain, even seriously affecting the patient&#39;s quality of life1. Starting rehabilitation as early as possible after an acute strain injury is the key to reducing the time to return to sports2&#160;and in reducing pain3,4. In modern Western medicine, clinical first aid for muscle injuries follows the principles of rest, ice, compression, and elevation (RICE) to stop injurious bleeding into the muscle tissue5&#160;and non-steroidal anti-inflammatory drugs to relieve pain6. The discovery of novel therapies such as exosomes7&#160;and tissue engineering8&#160;became potential treatment strategies for skeletal muscle diseases, compensating for previous pharmacological treatments&#39; shortcomings. However, it can also increase the cost of treatment for patients, putting them under tremendous financial pressure9. Therefore, alternative and complementary therapies are recommended for treating musculoskeletal problems10. Tuina is widely used clinically in China as a traditional medical method and is popular among patients for its efficacy and fewer side effects. Tuina therapy for musculoskeletal disorders can alleviate pain and improve function11,12,13. Mr. Ding Jifeng, a famous Shanghai Tuina practitioner, founded Ding&#39;s roll method14. It is a unique rolling and crushing technique with a large force area, uniform and gentle force, and intense penetration.
Different animal models are based on different etiologies. They have advantages and disadvantages, and the selection of correct and appropriate animal models is of great significance to basic experiments, which helps understand the cellular and molecular signaling pathways of regeneration and repair after skeletal muscle injury to develop new therapies for treating the treatment of skeletal muscle diseases. Chemically induced models of muscle injury are widely used, with injections of skeletal muscle causing myofiber necrosis and producing regenerated areas that can effectively regenerate within 2 weeks15. Both notexin and bupivacaine can cause muscle damage. However, notexin can cause more severe myotoxic damage to skeletal muscle than bupivacaine, and natural functional recovery is relatively slower16. Drug intramuscular injection molding not only takes less time but also has controlled effects and extent of skeletal muscle damage. This quantifiable control makes successful molding less difficult15,17.
Inflammatory response is an essential biological response that has been extensively studied in the context of myopathy18,19. In the early stages of skeletal muscle injury, myofiber necrosis disrupts local muscle homeostasis, and many inflammatory cells infiltrate the injury site, secreting many pro-inflammatory cytokines19. Creatine kinase (CK) is a traditional serum biomarker for assessing skeletal muscle injury. However, it lacks tissue specificity20&#160;and sensitivity21, which limits its ability to assess the extent of drug-induced muscle damage and indirectly report the extent of muscle recovery after injury. Novel biomarkers, including fatty acid binding protein 3 (FABP3), have recently shown relatively high tissue specificity and sensitivity in rodent models of skeletal muscle injury. FABP3 is a family of binding proteins expressed primarily in cardiac and skeletal muscle cells and implicated in fatty acid metabolism, transport, and signaling22. Therefore, we chose a combination of two biomarkers, CK and FABP3, to assess the extent of notexin-induced skeletal muscle damage and recovery after treatment.
In rodents, the muscles are shallow, and the skin area is small, which also determines that the various parameters of massage in rodents will not be the same as in humans, such as in animal therapy, the massage therapist should treat them with less force using Ding&#39;s roll method, and may not be conducive to the operation of this technique due to the small size of the injured area, which can ultimately lead to a reduction in the effectiveness of the massage. Therefore, the experiment utilized the rolling massager made in-house, which conforms to the characteristics of Ding&#39;s roll method, to intervene and evaluate the therapeutic effect of the notexin-induced skeletal muscle injury model in rats, which helps to standardize the parameters of Tuina in experimental animal studies in order to profoundly investigate the molecular mechanism of action of Tuina, a traditional Chinese medicine treatment method, on musculoskeletal diseases.

Autore in primo piano: Esecuzione del massaggio Tuina nei ratti utilizzando un massaggiatore autocostruito 

Imitazione del metodo di rotolamento di Ding sulla lesione muscolare indotta da Notexin nei ratti

The scope of our research is the biological mechanism of tuina in the treatment of musculoskeletal diseases. Here we are trying to make the self-made tuina device to standardize and effectively perform tuina treatments on laboratory animals. We are addressing the inability to perform this rolling manipulation with pairs by creating a massager that can simulate this rolling manipulation on rats, and verifying the effectiveness of the massager on rats with muscle injuries.We will focus on the questions of how efficacious the rolling massager is on the skeletal muscle injury in rats under different parameters in future standardized research.

Watch this Scientific Journal Video about Establishment of a Rat Model of Skeletal Muscle Injury at JoVE.com

Establishment of a Rat Model of Skeletal Muscle Injury  

Creazione di un modello di ratto di lesione del muscolo scheletrico

Inizia dividendo in modo casuale i ratti in tre gruppi di otto ratti ciascuno, inclusi i gruppi di controllo, notexina e notexina con tuina. Rimuovere i peli dall'arto inferiore destro con crema depilatoria dopo aver anestetizzato il ratto. Quindi disinfettare la pelle con tre cicli alternati di soluzione disinfettante iota four e alcol al 75% con un movimento circolare verso l'esterno.Per stabilire il modello di lesione del muscolo scheletrico, utilizzare una siringa da un millilitro con un ago calibro 30 per iniettare la soluzione di notexina per via intramuscolare nel muscolo gastrocnemio in una sola gamba. Attendere tre secondi prima di estrarre l'ago. Iniettare nei ratti del gruppo di controllo 200 microlitri di soluzione salina.Sposta i ratti anestetizzati in gabbie vuote con lettiera pulita. Osservare visivamente il colore dei tessuti e la frequenza respiratoria fino a quando i ratti non riacquistano sufficiente coscienza. Rimetti i ratti nella gabbia di casa e in genere allevali per 24 ore.Le proprietà morfologiche del muscolo scheletrico di ratto dopo la lesione sono state osservate mediante colorazione con ematossilina ed eosina. Il muscolo gastrocnemio danneggiato dall'iniezione di notexina ha mostrato la rottura di molte cellule muscolari rispetto ai controlli. Le cellule erano atrofiche, necrotiche e disposte in modo irregolare, con un'elevata infiltrazione di neutrofili e linfociti intorno all'area interessata.