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Please note that all translations are automatically generated. Click here for the English version.
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02:37 min
September 1st, 2023
DOI :
10.3791/200624-v
Transcript
首先,取准备好的人类粪便样本。将高速冷冻离心机冷却至4摄氏度。使用PBS将样品管的重量调节至0.1克,并在4摄氏度下以3, 000g离心20分钟。
使用塑料移液管,将上清液转移到两个 50 毫升管中,留下 1 毫升。然后,将上清液在12, 000 G下在4摄氏度下离心30分钟。使用20毫升注射器,吸出上清液,然后从注射器中取出针头。
通过 0.22 微米过滤器将液体过滤到 50 毫升管中。接下来,用 75% 的酒精擦拭桶以消除任何残留的污染。将大约30毫升过滤的粪便
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