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Please note that all translations are automatically generated. Click here for the English version.
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03:25 min
January 19th, 2024
DOI :
10.3791/200627-v
Transcript
首先,用75%酒精喷洒安乐死的小鼠。然后你消毒解剖剪刀,打开皮肤,露出胸腔。切开横膈膜,然后继续向上切至下颌,小心地去除多余的结缔组织和暴露颈动脉的脂肪。
接下来,切开下腔静脉,使血液从闭合的循环中流出。使用注射器将 20 毫升预冷灌注溶液缓慢注入左心室。将鼠标放在立体显微镜下。
然后用显微解剖剪刀,剥离颈动脉周围的脂肪和结缔组织。分离颈动脉并用PBS清洗,以冲洗掉任何残留的血液。将动脉转移到冰上含有1.5%FBS的六孔板中。
使用显微解剖剪刀,纵向解剖
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