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Please note that all translations are automatically generated. Click here for the English version.
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01:03 min
August 18th, 2023
DOI :
10.3791/200631-v
Transcript
要创建蛛网膜下腔出血或SAH模型,将显微注射针插入麻醉的大鼠鞍上腔。用注射泵将从大鼠尾动脉获得的自体血注入鞍上蓄水池。现在将另一根针头植入给定坐标处的右外侧脑室。
对于 SAH 模型,注射 0.2 毫升大鼠尾动脉血。对于SHAM组,注射相同体积的等渗盐水。斩首后,提取大鼠的整个大脑,并将它们浸入冰冷的人造脊髓液或ACSF中。
用振动切片机准备200微米的急性脑切片和冰冷平衡的ACSF。将脑切片放在尼龙网上,浸入 35 至 37 摄氏度储存室中的平衡 ACSF 中。<
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